【IL 13】重組人白介素-13
產(chǎn)品報價
¥800/2μg ¥2080/10μg ¥74880/1mg
背景資料
IL13 is an immunoregulatory cytokine produced primarily by activated Th2 cells. IL-13 is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4.
產(chǎn)品描述
人白介素-13重組蛋白是在大腸桿菌中重組表達的,是一條單鏈、非糖基化的多肽鏈,包含有112個氨基酸,分子量為12kDa。該人白介素-13重組蛋白最終由標準的色譜技術(shù)純化出。
來源宿主
大腸桿菌中重組表達
序列
GPVPPSTALRELIEELVNITQNQKAPLCNGSMVWSINLTAGMYCAALESLINVS GCSAIEKTQRMLSGFCPHKVSAGQFSSLHVRDTKIEVAQFVKDLLLHLKKLFRE GRFN.
溶解建議
提供的人白介素-13(IL 13)產(chǎn)品為過濾滅菌的白色凍干粉末;該凍干粉末是從含1mg/ml人白介素-13(IL 13)的1X PBS(pH 7.2)溶液中凍干產(chǎn)生的。我們建議加無菌的18MΩ-cm水以配制工作儲存液到100μg/ml,后繼實驗過程中可根據(jù)實驗要求進一步稀釋。
保存建議
人白介素-13(IL 13)在室溫可保存3周,或置于-18℃凍存用于長期保存。重新溶解后可以在4℃冷藏保存至少2-7天;如果想延長保存時間,建議分裝(加載體蛋白如0.1% HSA或 BSA)后置于-18℃凍存。請避免反復(fù)凍融。
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